Composite
Part:BBa_K2259071:Design
Designed by: Laurynas Karpus Group: iGEM17_Vilnius-Lithuania (2017-10-02)
SynORI constitutive plasmid copy number device (Anderson 1.0)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 685
Illegal NheI site found at 708 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A mutation was made to avoid prohibited restriction sites.
Source
Taken from wildtype pSK41 plasmid found in genbank.